Jump to main content
Jump to site search

Issue 20, 2017
Previous Article Next Article

Cellular uptake of pH/reduction responsive phosphorylcholine micelles

Author affiliations


Phosphorylcholine micelles based on pH/reduction responsive copolymers, poly(ε-caprolactone)-ss-b-poly((N,N-diethylaminoethyl methacrylate)-r-poly(2-methacryloyloxyethyl phosphorylcholine)) (PCL-ss-PDEAPMPC) were developed for the intracellular delivery of doxorubicin. The micelles were spherical (less than 90 nm in diameter) and demonstrated pH/reduction sensitivity. The DOX loaded micelles presented the fastest drug release under simultaneously acidic and reductive conditions. Although PCL20-ss-PDEA5PMPC10 had lower pH sensitivity than PCL20-ss-PDEA15PMPC10, the DOX loaded micelles of the former released the drug faster than the latter at pH 5.0 and in the presence of a reductant. The cytotoxicities of the blank micelles and the drug-loaded micelles were investigated using the human cervical cancer cell line (HeLa). The IC50 (half maximal inhibitory concentration) of PCL20-ss-PDEA5PMPC10 micelles was the lowest among PCL-ss-PDEAPMPC micelles. The results of CLSM and flow cytometry showed that the micelles effectively delivered the drug cargo into cancer cells. The cellular uptake pathways of PCL-ss-PDEAPMPC micelles were mainly clathrin-mediated endocytosis, and accompanied by a certain degree of giant pinocytosis.

Graphical abstract: Cellular uptake of pH/reduction responsive phosphorylcholine micelles

Back to tab navigation

Supplementary files

Publication details

The article was received on 10 Jul 2017, accepted on 29 Aug 2017 and first published on 29 Aug 2017

Article type: Paper
DOI: 10.1039/C7NJ02484C
Citation: New J. Chem., 2017,41, 11828-11838
  •   Request permissions

    Cellular uptake of pH/reduction responsive phosphorylcholine micelles

    Y. Cai, S. Li, M. Cai, Y. Chen and X. Luo, New J. Chem., 2017, 41, 11828
    DOI: 10.1039/C7NJ02484C

Search articles by author