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Cu2+-Mediated turn-on fluorescence assay for sulfide ions using glutathione-protected gold nanoclusters: enhanced sensitivity, good reusability, and cell imaging

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Abstract

We develop a simple assay for highly selective and sensitive fluorescence detection of sulfide ions (S2−) using glutathione-protected gold nanoclusters (GSH-Au NCs). This approach was based on the much higher affinity of CuS than that of the Cu–GSH complex, which makes the fluorescence of the NCs quenched by Cu2+ recover upon addition of S2−. Importantly, the assistance of Cu2+ enables S2− detection in the “turn-on” mode, eventually enhancing detection sensitivity toward S2− about 15 times more compared to that in the “turn-off” mode. Under the optimal conditions, the linear fluorescence response toward S2− was given within the concentration range of 2 to 24 μM with a low detection limit of 0.7 μM. Also, the GSH-Au NCs show good reusability for S2− detection due to effective removal of the bridging reagent Cu2+ by S2−. This phenomenon is further exploited as an integrated logic gate and successfully employed for imaging S2− in living cells.

Graphical abstract: Cu2+-Mediated turn-on fluorescence assay for sulfide ions using glutathione-protected gold nanoclusters: enhanced sensitivity, good reusability, and cell imaging

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Publication details

The article was received on 08 Jul 2017, accepted on 13 Sep 2017 and first published on 13 Sep 2017


Article type: Paper
DOI: 10.1039/C7NJ02465G
Citation: New J. Chem., 2017, Advance Article
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    Cu2+-Mediated turn-on fluorescence assay for sulfide ions using glutathione-protected gold nanoclusters: enhanced sensitivity, good reusability, and cell imaging

    S. Li, J. Feng, P. Huang and F. Wu, New J. Chem., 2017, Advance Article , DOI: 10.1039/C7NJ02465G

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