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A turn-on fluorescence aptasensor based on carbon dots for sensitive detection of adenosine

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Abstract

In this paper, a novel turn-on fluorescence aptasensor has been designed for adenosine detection based on fluorescence resonance energy transfer (FRET) from single-stranded DNA labeled carbon dots (ssDNA-CDs) to aptamer modified gold nanoparticles (aptamer-AuNPs). In the absence of adenosine, the fluorescence of ssDNA-CDs was quenched by aptamer-AuNPs via the formation of an aptamer–ssDNA duplex. The introduced adenosine competed to displace ssDNA-CDs by specifically binding to the aptamer, resulting in the recovery of the quenched fluorescence of ssDNA-CDs. Under optimized conditions, the increase in fluorescence intensity was proportional to the concentration of adenosine with a linear range of 10–500 nM and allowed a limit of detection as low as 4.2 nM. In addition, this method was successfully applied to adenosine determination in human serum samples with satisfactory results.

Graphical abstract: A turn-on fluorescence aptasensor based on carbon dots for sensitive detection of adenosine

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Publication details

The article was received on 03 Jul 2017, accepted on 18 Jul 2017 and first published on 19 Jul 2017


Article type: Paper
DOI: 10.1039/C7NJ02384G
Citation: New J. Chem., 2017, Advance Article
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    A turn-on fluorescence aptasensor based on carbon dots for sensitive detection of adenosine

    X. Shen, L. Xu, W. Zhu, B. Li, J. Hong and X. Zhou, New J. Chem., 2017, Advance Article , DOI: 10.1039/C7NJ02384G

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