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Colorimetric detection of heparin with high sensitivity based on the aggregation of gold nanoparticles induced by polymer nanoparticles

Abstract

Herein, a new colorimetric strategy based on polymer nanoparticles (PNPs) and Au nanoparticles (AuNPs) is developed to detect heparin (Hep). The cationic PNPs can directly induce the aggregation of AuNPs, which are stabilized by negatively charged coating of citrate ions, so the absorption peak of AuNPs moves to 675 nm accompanying with the color change from wine red to blue. However, the negatively charged Hep will compete with AuNPs and binds to PNPs, hindering the interaction between PNPs and AuNPs. As a result, the absorption band of AuNPs blue shifts and the color turns to red again. The reaction can be completed within 10 min, and the color variation produced by 150 nM of Hep can be distinguished by naked eyes. Besides, this strategy also shows good selectivity and sensitivity, and the linear range of Hep is from 10 to 400 nM with detection limit of 2.5 nM. Finally, this colorimetric assay has been applied to measure Hep in Hep sodium injection and human serum samples with satisfactory recoveries.

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Publication details

The article was received on 03 Jul 2017, accepted on 09 Aug 2017 and first published on 11 Aug 2017


Article type: Paper
DOI: 10.1039/C7NJ02381B
Citation: New J. Chem., 2017, Accepted Manuscript
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    Colorimetric detection of heparin with high sensitivity based on the aggregation of gold nanoparticles induced by polymer nanoparticles

    F. Qu, Y. Liu, H. Lao, Y. Wang and J. You, New J. Chem., 2017, Accepted Manuscript , DOI: 10.1039/C7NJ02381B

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