Issue 17, 2017

Polyglycolide–montmorillonite as a novel nanocomposite platform for biosensing applications

Abstract

In catalytic biosensors, the immobilization of biomolecules in a suitable matrix is one of the vital parameters for obtaining improved systems. Clays, which are intercalated with various organic compounds, have a great tendency to develop biosensors with high stability, sensitivity and reproducibility. Herein, a polymer/clay nanocomposite based on natural silicate montmorilonite (Mt) and a biodegradable polymer polyglycolide (PGA) was prepared and characterized by FT-IR, thermogravimetric analysis, differential thermogravimetric analysis and X-ray diffraction. Then, the resulting matrix was used as a fixation matrix for pyranose oxidase (POx), which was selected as a model enzyme. The bioactive layer was fabricated by immobilization of POx on glassy carbon electrodes by means of PGA–Mt and bovine serum albumin. The POx biosensor revealed a good linear range from 0.01 to 0.5 mM glucose with a LOD of 1.2 μM. After the optimization of the working and preparation conditions, characterization studies were performed for glucose detection. Finally, the PGA–Mt/POx biosensor was confirmed to have detected glucose in beverages without needing any sample pre-treatment.

Graphical abstract: Polyglycolide–montmorillonite as a novel nanocomposite platform for biosensing applications

Article information

Article type
Paper
Submitted
19 May 2017
Accepted
24 Jul 2017
First published
25 Jul 2017

New J. Chem., 2017,41, 9371-9379

Polyglycolide–montmorillonite as a novel nanocomposite platform for biosensing applications

B. Unal, E. E. Yalcinkaya, S. Gumustas, B. Sonmez, M. Ozkan, M. Balcan, D. O. Demirkol and S. Timur, New J. Chem., 2017, 41, 9371 DOI: 10.1039/C7NJ01751K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements