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Morphological and in vitro evaluation of programmed cell death in MCF-7 cells by new organoruthenium(II) complexes

Abstract

Cyclopentadienylruthenium(II) thiosemicarbazone complexes, with the general formula [Ru(η5-C5H5)(Ac-tsc)PPh3].Cl (1), [Ru(η5-C5H5)(Ac-mtsc)PPh3].Cl (2), [Ru(η5-C5H5)(Ac-etsc)PPh3].Cl (3) and [Ru(η5-C5H5)(Ac-ptsc)PPh3] (4) were synthesized and characterized by various spectroscopic techniques (1H-NMR, 13C-NMR, IR and UV-Vis). The molecular structure of representative complexes 2 and 4 was studied by single crystal X-Ray diffraction. Interaction of all the ligands and complexes with Calf Thymus-DNA (CT-DNA) and Bovine Serum Albumin (BSA) was studied using UV-Vis and Fluorescence emission spectroscopy, the results of the binding studies cleared that the effective binding potential of the complexes are higher than their parent ligands. All the new complexes (1-4) were evaluated for their in vitro cytotoxic activity against MCF-7 cancer cell line. All the complexes significantly inhibited cell proliferation in human MCF-7 breast cancer cells in a dose-dependent manner. Cytological observations by an inverted phase contrast microscope and Hoechst 33342/PI dual-staining assay showed typical apoptotic morphology of cancer cells upon treatment with complexes 2 and 3. It can thus be suggested that complexes 2 and 3 are modulated by apoptosis. The findings of the present study support that the complexes 2 and 3 can be of potent drugs for the treatment of cancer related diseases only after further explorations.

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Publication details

The article was received on 17 May 2017, accepted on 03 Jul 2017 and first published on 03 Jul 2017


Article type: Paper
DOI: 10.1039/C7NJ01707C
Citation: New J. Chem., 2017, Accepted Manuscript
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    Morphological and in vitro evaluation of programmed cell death in MCF-7 cells by new organoruthenium(II) complexes

    G. Devagi, F. Reyhaneh, F. Dallemer, R. Jayakumar, K. Palaniappan and R. Prabhakaran, New J. Chem., 2017, Accepted Manuscript , DOI: 10.1039/C7NJ01707C

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