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Simultaneous Detection of Clenbuterol and Ractopamine Basing on Multiplexed Competitive Surface Enhanced Raman Scattering (SERS) Immunoassay

Abstract

In recent years, the illegal abuse of β2-adrenergic agonists in livestock for growth promotion has been reported frequently. For conventional immunoassay methods, the development of quantitative and simultaneous detection is a great challenge. In this report, a highly sensitive multiplexed immunoassay based on competitive surface-enhanced Raman scattering (SERS) spectroscopy has been developed for the detection of clenbuterol and ractopamine simultaneously. A SERS nanoprobe based on gold nanoparticles (AuNPs) was attached by 4,4´-dipyridyl (DP), clenbuterol antibody and 2,2´-dipyridy (BP), ractopamine antibody respectively. The detections of multiple antibodies were implemented by competitive binding between free clenbuterol, free ractopamine and clenbuterol-BSA, ractopamine-BSA fastened on the substrates with their antibody labeled on SERS nanoprobes. The results indicated that the proposed method can detect clenbuterol and ractopamine simultaneously over a wide concentration range (1-1000 pg/mL) with a lower limit of ca.1.0 pg/mL than conventional methods,which holds great potential for applications in biochemical analysis and food safety.

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Publication details

The article was received on 26 Apr 2017, accepted on 07 Aug 2017 and first published on 07 Aug 2017


Article type: Paper
DOI: 10.1039/C7NJ01394A
Citation: New J. Chem., 2017, Accepted Manuscript
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    Simultaneous Detection of Clenbuterol and Ractopamine Basing on Multiplexed Competitive Surface Enhanced Raman Scattering (SERS) Immunoassay

    M. Yu, Y. Hu and J. Liu, New J. Chem., 2017, Accepted Manuscript , DOI: 10.1039/C7NJ01394A

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