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Dichlorofluorescein as a peroxidase mimic and its application to glucose detection

Abstract

In this article, we discovered for the first time that 2′,7′-dichlorofluorescein (DCF) as a novel peroxidase mimic can catalyze the oxidation of classical peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2, producing a colour reaction. While a variety of peroxidase mimics have been actively developed recently, few study has been made towards the small molecule peroxidase mimics. As a novel peroxidase mimic, DCF was verified to be highly stable, well reproducible and of low cost, exhibiting typical Michealis-Menten kinetics, and showed strong affinity towards H2O2 and TMB. On the basis of these findings, we further demonstrated the novel use of DCF for easy and simple detection of H2O2 and glucose. This kind of new small molecule peroxidase mimic is expected to be used in medical clinical diagnosis, and this novel finding not only further confirm the peroxidase activity of small molecules of fluorescein derivatives, but also open new opportunities to deepen the knowledge of the new class of small molecule enzyme.

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Publication details

The article was received on 12 Apr 2017, accepted on 17 Jun 2017 and first published on 19 Jun 2017


Article type: Paper
DOI: 10.1039/C7NJ01213F
Citation: New J. Chem., 2017, Accepted Manuscript
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    Dichlorofluorescein as a peroxidase mimic and its application to glucose detection

    M. Li, L. Liu, Y. Shi, Y. Yang, H. Zheng and Y. Long, New J. Chem., 2017, Accepted Manuscript , DOI: 10.1039/C7NJ01213F

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