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A β-diketonate–europium(III) complex-based fluorescent probe for highly sensitive time-gated luminescence detection of copper and sulfide ions in living cells

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Abstract

We have developed a novel β-diketonate–europium(III) complex-based fluorescent probe, Eu3+-BHHCT-BPED, by introducing Cu2+-binding dipicolylamine (DPA)-containing N,N-bis(2-pyridylmethyl)ethanediamine (BPED) into a tetradentate β-diketone ligand 4,4′-bis(1′′,1′′,1′′,2′′,2′′,3′′,3′′-heptafluoro-4′′,6′′-hexanedione-6′′-yl)chlorosulfo-o-terphenyl (BHHCT). The probe was strongly fluorescent with a high quantum yield (57.6%) and a long luminescence lifetime (0.81 ms). Upon addition of Cu2+ ions, the fluorescence emission of Eu3+-BHHCT-BPED at 607 nm was selectively and efficiently quenched due to the coordination of Cu2+ ions with the BPED moiety. Furthermore, the resulting heterobimetallic complex Eu3+-BHHCT-BPED-Cu2+ displayed specific luminescence response to sulfide ions because a reversible fluorescence revival was induced by the formation of CuS. The “on–off–on” type signaling behavior of Eu3+-BHHCT-BPED towards Cu2+ and sulfide ions enabled the sensing of the two species with the detection limits of 3.7 nM and 0.19 μM, respectively. The time-gated luminescence imaging study has demonstrated the applicability of Eu3+-BHHCT-BPED for monitoring intracellular Cu2+ and sulfide ions with high sensitivity and selectivity.

Graphical abstract: A β-diketonate–europium(iii) complex-based fluorescent probe for highly sensitive time-gated luminescence detection of copper and sulfide ions in living cells

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Publication details

The article was received on 10 Mar 2017, accepted on 24 May 2017 and first published on 24 May 2017


Article type: Paper
DOI: 10.1039/C7NJ00802C
Citation: New J. Chem., 2017, Advance Article
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    A β-diketonate–europium(III) complex-based fluorescent probe for highly sensitive time-gated luminescence detection of copper and sulfide ions in living cells

    Y. Wang, H. Wang, X. Zhao, Y. Jin, H. Xiong, J. Yuan and J. Wu, New J. Chem., 2017, Advance Article , DOI: 10.1039/C7NJ00802C

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