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Synthesis of mesoporous silica with different pore size for cellulase immobilization: pure physical adsorption

Abstract

To discuss the physical adsorption mechanism of the adsorption process of cellulase, a commercial enzyme cocktail sauced from Acremonium, was immobilized in mesoporous silica with various pore sizes by pure physical adsorption in this study. Mesoporous silica materials with 17.6 nm and 3.8 nm pore size (hereafter referred to as Ms-17.6nm and Ms-3.8nm, respectively) were synthesized in the manner of a seeded-growth method. Other available mesoporous silica denoted as H-32 and diatomite were also used as sorbents. Then the sorbents were characterized via Small-angle X-ray scattering (SAXS), transmission electron microscope (TEM), scanning electron microscope (SEM), Barrett-Emmett-Teller (BET) method and Brarrett-Joyner-Halanda (BJH) method to confirm their mesostucture. Furthermore, adsorption ability of different sorbents and enzymatic activities of immobilized cellulase were studied. The adsorption amounts exhibited a clear correlation with the pore size of the sorbents; i.e., the adsorption amounts of MS-17.6nm (410 mg/g) with the pore size was similar to the long axes of cellulase molecule was higher than that for MS-3.8nm (315 mg/g) with the pore size approximated to the short axes of cellulase (which was realized at 50 °C). Besides, the adsorption behavior of diatomite (with pore size about 200 nm) revealed a periodicity because the pore size was significantly larger than cellulase molecules. In the meantime, the pore size was suggested to be a critical factor for enzymatic activity of the cellulase. When the average pore size of MS-3.8nm just matched the short axes of cellulase molecules, immobilized cellulase preserved active sites of cellulase intactly and showed the best activity (i.e. 63.3% of free cellulase activity at 50 °C). Consequently, pore size of the sorbents had a significant influence on cellulase immobilization.

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Publication details

The article was received on 08 Feb 2017, accepted on 13 Jul 2017 and first published on 14 Jul 2017


Article type: Paper
DOI: 10.1039/C7NJ00441A
Citation: New J. Chem., 2017, Accepted Manuscript
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    Synthesis of mesoporous silica with different pore size for cellulase immobilization: pure physical adsorption

    B. Y. Chen, J. Qiu, H. Mo, Y. Yu, K. Ito, E. sakai and H. X. Feng, New J. Chem., 2017, Accepted Manuscript , DOI: 10.1039/C7NJ00441A

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