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Poly (glutamic acid) Hydrogels crosslinked via Native Chemical Ligation


Mild crosslinking methods, which have strong influence to biomedical hydrogels and scaffolds, have attracted wild attention in recent years. In this project, native chemical ligation (NCL) was utilized to prepare biocompatible and biodegradable hydrogel using naturally derived poly (glutamic acid) (PGA) without any additive and byproduct. First, thiolactone grafted poly (glutamic acid) (PGA-HC) and cysteine grafted poly (glutamic acid) (PGA-C) precursors were synthesized. Their structure was confirmed by nuclear magnetic resonance (NMR). Then, NCL crosslinked hydrogel was formed by blending PGA-HC and PGA-C buffer solutions without any additives under physiological condition. After that, the equilibrium water content, morphology, degradation rate and mechanical properties of the hydrogels were characterized in detail. The data showed the PGA hydrogels had tunable gelation time, water content and mechanical property by adjusting precursor composition. Furthermore, the biocompatibility of hydrogels was confirmed by MTT assay. These characteristics provide a potential opportunity for the NCL hydrogels as wound dressing, skin fillings, drug delivery vehicles and tissue regeneration matrix.

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Publication details

The article was received on 07 Feb 2017, accepted on 08 Jul 2017 and first published on 10 Jul 2017

Article type: Paper
DOI: 10.1039/C7NJ00439G
Citation: New J. Chem., 2017, Accepted Manuscript
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    Poly (glutamic acid) Hydrogels crosslinked via Native Chemical Ligation

    Z. Fan, P. Cheng, M. Liu, D. Li, G. Liu, Y. Zhao, Z. Ding, F. Chen, B. Wang, X. Tan, Z. Wang and J. Han, New J. Chem., 2017, Accepted Manuscript , DOI: 10.1039/C7NJ00439G

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