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Issue 13, 2017
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Fabrication of imidazolium-functionalized magnetic composite microspheres for selective recognition and separation of heme proteins

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Abstract

In this work, a facile route was presented to fabricate magnetic composite microspheres composed of a high-magnetic-response magnetic microsphere Fe3O4 core and an imidazolium-modified poly 4-vinylbenzylchloride (pVBC) shell via distillation–precipitation polymerization. The applicability of the as-synthesized Fe3O4@SiO2@pVBC@IL magnetic composite microspheres (MMPs) was investigated in the selective recognition and separation of heme proteins mainly based on the covalent coordination between the ferrous ion in the heme group of the heme proteins and the imidazolium cation. The experimental results showed that Fe3O4@SiO2@pVBC@IL MMPs exhibited high hemoglobin binding capacity (6321 mg g−1), which was due to the pure pVBC@IL interface and high imidazolium ion loading amount, and high efficiency and excellent selectivity in the isolation of hemoglobin from a protein mixture and human blood samples. These good results demonstrate that the prepared MMPs could be applied in the removal of high abundant protein (heme proteins) from samples before proteomic analysis.

Graphical abstract: Fabrication of imidazolium-functionalized magnetic composite microspheres for selective recognition and separation of heme proteins

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Publication details

The article was received on 09 Jan 2017, accepted on 18 May 2017 and first published on 05 Jun 2017


Article type: Paper
DOI: 10.1039/C7NJ00109F
Citation: New J. Chem., 2017,41, 5651-5659
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    Fabrication of imidazolium-functionalized magnetic composite microspheres for selective recognition and separation of heme proteins

    B. Wang, Q. Shao, Y. Fang, J. Wang, X. Xi, Q. Chu, G. Dong and Y. Wei, New J. Chem., 2017, 41, 5651
    DOI: 10.1039/C7NJ00109F

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