A mitochondria-targetable fluorescent probe with a large Stokes shift for detecting hydrogen peroxide in aqueous solution and living cells
Fluorescent probes with large Stokes shifts play a key role in maintaining the accuracy of biological imaging and minimizing the self-quenching effect. In this work, we have developed a novel fluorescent probe CAI, which possessed a large Stokes shift in various solutions. We found that CAI was capable of detecting hydrogen peroxide (H2O2) in aqueous solution. Furthermore, probe CAI can sense exogenous and endogenous H2O2 in the mitochondria of living RAW 264.7 cells. In addition, probe CAI exhibited ideal properties such as excellent photostability, high sensitivity and selectivity. This fluorescent dye with a large Stokes shift was expected to have a more broad range of applications for developing various fluorescent probes with large Stokes shifts.