Jump to main content
Jump to site search


Pyrene-Fluorescein based colour-tunable AIE active hybrid fluorophore material for potential live cell imaging application

Abstract

A novel hybrid fluorophore (FHPY) has been synthesized based on condensation reaction of two standard fluorescent hydrophobic-hydrophilic molecules viz. pyrene and fluorescein with an objective to tune the aggregation induced emission (AIE) along with morphology. Owing to distinct photophysical properties of pyrene and fluorescein, the hybrid FHPY dramatically exhibits the flourescence change from colourless to yellow-green via blue colour upon varying the volume fraction of water (poor solvent) in methanol (good solvent). FHPY has not been only exhibited AIE, but also outstanding quantum yield (F) 97% at 70% water fraction in methanol (70:30, v/v). We attribute the reason behind tuning of AIE and quantum yield to the opening of lactam ring of fluorescein as well as the amassing of hydrophobic pyrene at certain water fraction. The mechanism involved in AIE has been well supported by a detailed UV-vis, fluorescence, lifetime, SEM, AFM, DFT, PXRD and 1H NMR experiments. In addition, FHPY served as good candidate for live cell imaging process of HeLa cells.

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 09 Jan 2017, accepted on 12 May 2017 and first published on 17 May 2017


Article type: Paper
DOI: 10.1039/C7NJ00106A
Citation: New J. Chem., 2017, Accepted Manuscript
  •   Request permissions

    Pyrene-Fluorescein based colour-tunable AIE active hybrid fluorophore material for potential live cell imaging application

    V. K. Singh, R. Prasad, B. Koch, S. H. Hasan and M. Dubey, New J. Chem., 2017, Accepted Manuscript , DOI: 10.1039/C7NJ00106A

Search articles by author