Jump to main content
Jump to site search


Phenolic compound-mediated single-step fabrication of copper oxide nanoparticles for elucidating their influence on anti-bacterial and catalytic activity

Author affiliations

Abstract

A single-step synthesis of copper oxide nanoparticles (CuO NPs) from a phenolic compound (rosmarinic acid) at ambient temperature was developed in this study. The physico-chemical properties of the synthesized particles were characterized by different instruments (UV-visible; ultra-violet spectroscopy, XRD; X-ray diffraction, FTIR; Fourier transformed infrared resonance, FESEM; field emission scanning electron microscope equipped with EDS; energy dispersive spectroscopy and TEM; transmission electron microscope), which revealed the good crystallinity with homogeneous distribution (30 ± 5 nm) of CuO NPs on the surface. The applicability of the nanoparticles as an anti-bacterial agent against Gram-negative (E. coli) and Gram-positive (S. aureus) bacterial strain was determined by bactericidal kinetics study. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values as determined by a batch culture process that exhibited a considerably low amount of copper content are capable of enhancing the anti-microbial efficacy. The cyto-compatibility of the CuO NPs was investigated by cell viability (MTT) and proliferation of mammalian fibroblast cell line experiments. The underlying mechanisms behind the anti-microbial activity and cell death were also studied by inner membrane permeabilization assay (β-galactosidase assay), intracellular ROS measurement, fluorescence images and cell wall degradation by FESEM. Significant photocatalytic activity of CuO NPs was observed through the degradation of methylene blue (MB) and para-nitrophenol (PNP) under visible light exposure conditions. The phenolic compound-assisted CuO NPs have the advantages of being environmentally benign with a beneficial and single-step synthesis approach that can be further used for various biomedical and waste water treatment purposes.

Graphical abstract: Phenolic compound-mediated single-step fabrication of copper oxide nanoparticles for elucidating their influence on anti-bacterial and catalytic activity

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 20 Dec 2016, accepted on 26 Apr 2017 and first published on 26 Apr 2017


Article type: Paper
DOI: 10.1039/C6NJ04008J
Citation: New J. Chem., 2017, Advance Article
  •   Request permissions

    Phenolic compound-mediated single-step fabrication of copper oxide nanoparticles for elucidating their influence on anti-bacterial and catalytic activity

    N. Bala, M. Sarkar, M. Maiti, P. Nandy, R. Basu and S. Das, New J. Chem., 2017, Advance Article , DOI: 10.1039/C6NJ04008J

Search articles by author