A nitroolefin functionalized DPP fluorescent probe for selective detection of hydrogen sulfide
In this paper, a nitroolefin functionalized probe DPP-NO2 was designed and synthesized as a selective fluorescent probe for detection of hydrogen sulfide (H2S). The specific reduction reaction between nitro group and H2S gave 800-fold fluorescence turn-on at 550 nm in the fluorescence emission spectrum, along with a 21 nm blue-shift from 506 nm to 485 nm in the absorption spectrum as well as the color change from pink to light yellow. In addition, high selectivity, fast response (3 minutes) and low detection limit of 5.2 nM of the probe DPP-NO2 for H2S detection was observed. What is more, through the fluorescence turn-on signal toward H2S in Hela cells, probe DPP-NO2 was successfully applied to monitor the levels of endogenous and exogenous H2S in living cells.