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Issue 11, 1997
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Determination of Trimethoprim in Tissues Using Liquid Chromatography–Thermospray Mass Spectrometry

Abstract

A method is described for the determination of the antibacterial drug trimethoprim in tissues. Minced tissue is homogenised with chloroform–acetone (1 + 1 v/v), filtered, and the filtrate evaporated to an oily residue using a rotary evaporator. The residue is redissolved in methanol–water–acetic acid (50 + 48.7 + 1.3 v/v) and any fats present are partitioned into hexane. The aqueous phase is analysed by liquid chromatography–thermospray mass spectrometry in positive mode with the protonated molecular ion at m/z 291 being monitored. Recoveries ranged between 60% in liver and 79% in muscle. The limit of determination was 25 µg kg1 and the limit of detection was approximately 4 µg kg1. The method is suitable for monitoring tissues taken under national surveillance schemes for veterinary drug residues.

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Article type: Paper
DOI: 10.1039/A704348A
Citation: Analyst, 1997,122, 1379-1382
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    Determination of Trimethoprim in Tissues Using Liquid Chromatography–Thermospray Mass Spectrometry

    A. Cannavan, S. Armstrong Hewitt, S. D. Floyd and D. Glenn Kennedy, Analyst, 1997, 122, 1379
    DOI: 10.1039/A704348A

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