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The CO dehydrogenase accessory protein CooT is a novel nickel-binding protein


In Rhodospirillum rubrum, the maturation of Carbon Monoxide Dehydrogenase (CODH) requires three accessory proteins, CooC, CooT and CooJ, dedicated to nickel insertion into the active site, constituted of a distorted [NiFe3S4] cubane coordinated to a mononuclear Fe site. CooC is an ATPase proposed to provide the energy required for the maturation proces s, while CooJ is described as a metallochaperone with 16 histidines and 2 cysteines at the C-terminus, likely involved in metal binding and/or storage. Prior to the present study, no information was available on CooT at the molecular level. Here, the X-ray structure of RrCooT was obtained and revealed that this protein is a homodimer featuring a fold that resembles a Sm-like domain, suggesting a role in RNA metabolism that was otherwise not supported by experimental observations. Biochemical and biophysical evidences based on circular dichroism spectroscopy, light scattering, isothermal titration calorimetry and site-directed mutagenesis showed that RrCooT binds specifically a single Ni(II) per dimer, with a dissociation constant of 9 nM, through the pair of Cys2, a highly conserved residue, located at the dimer interface. Despite its role in the activation of RrCODH in vivo, CooT was thought to be a unique protein, found only in R. rubrum, with an unclear function. In this study, we extended the biological impact of CooT, establishing that this protein is a member of a novel Ni(II)-binding protein family with 111 homologues, linked to anaerobic metabolism in bacteria and archaea, and in most cases to the presence of CODH.

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Publication details

The article was received on 04 Mar 2017, accepted on 10 Apr 2017 and first published on 18 Apr 2017

Article type: Paper
DOI: 10.1039/C7MT00063D
Citation: Metallomics, 2017, Accepted Manuscript
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    The CO dehydrogenase accessory protein CooT is a novel nickel-binding protein

    J. Timm, C. Brochier-Armanet, J. Perard, B. Zambelli, S. Ollagnier de Choudens, S. Ciurli and C. Cavazza, Metallomics, 2017, Accepted Manuscript , DOI: 10.1039/C7MT00063D

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