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Issue 8, 2017
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Analytical approaches for the characterization of nickel proteome

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Abstract

The use of nickel in modern industry and in consumer products implies some health problems for the human being. Nickel allergy and nickel carcinogenicity are well-known health effects related to human exposure to nickel, either during production of nickel-containing products or by direct contact with the final item. In this context, the study of nickel toxicity and nickel carcinogenicity involves the understanding of their molecular mechanisms and hence the characterization of the nickel-binding proteins in different biological samples. During the last 50 years, a broad range of analytical techniques, covering from the first chromatographic columns to the last generation mass spectrometers, have been used in order to fully characterize the nickel proteome. The aim of this review is to present a critical view of the different analytical approaches that have been applied for the purification, isolation, detection and identification of nickel-binding proteins. The different analytical techniques used are discussed from a critical point of view, highlighting advantages and limitations.

Graphical abstract: Analytical approaches for the characterization of nickel proteome

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Publication details

The article was received on 24 Feb 2017, accepted on 26 Jun 2017 and first published on 06 Jul 2017


Article type: Critical Review
DOI: 10.1039/C7MT00054E
Citation: Metallomics, 2017,9, 1014-1027
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    Analytical approaches for the characterization of nickel proteome

    J. Jiménez-Lamana and J. Szpunar, Metallomics, 2017, 9, 1014
    DOI: 10.1039/C7MT00054E

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