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Issue 8, 2017
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A new gallium complex inhibits tumor cell invasion and matrix metalloproteinase MMP-14 expression and activity

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Abstract

In this study, we investigated the effect of [N-(5-chloro-2-hydroxyphenyl)-L-aspartato] chlorogallate (GS2), a new water soluble gallium complex, on cell invasion and on the expression and activity of matrix metalloproteinases (MMPs) in human metastatic HT-1080 fibrosarcoma and MDA-MB 231 breast carcinoma cells. The effect on cell invasion was studied using a modified Boyden chamber coated with a type-I collagen. We analyzed the effect of GS2 on MMP-2, MMP-9, and MMP-14 via zymography and enzymatic assay using high affinity fluorogenic substrates. The expression of MMP mRNA was analyzed via qRT-PCR. GS2 induced a decrease in cell invasion. A dose-dependent inhibition effect was observed on the activities of MMP-2, MMP-9, and MMP-14 with the IC50 values of 168, 82, and 20 μM, respectively. A decrease in the expression of MMP-14 mRNA was observed in both cell lines, whereas the expression of MMP-2 and MMP-9 mRNA was decreased only in the MDA-MB231 cells. Data obtained for the expression of MMP-14 mRNA were confirmed via Western blotting. In fact, MMP-14 expression was decreased in the presence of GS2. Overall, these data show that GS2 is a promising compound for anti-invasive and anticancer therapy.

Graphical abstract: A new gallium complex inhibits tumor cell invasion and matrix metalloproteinase MMP-14 expression and activity

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Publication details

The article was received on 20 Feb 2017, accepted on 18 Jul 2017 and first published on 18 Jul 2017


Article type: Paper
DOI: 10.1039/C7MT00049A
Citation: Metallomics, 2017,9, 1176-1184
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    A new gallium complex inhibits tumor cell invasion and matrix metalloproteinase MMP-14 expression and activity

    A. Mohsen, P. Collery, R. Garnotel, B. Brassart, N. Etique, G. Mohamed Sabry, R. Elsherif Hassan, P. Jeannesson, D. Desmaële and H. Morjani, Metallomics, 2017, 9, 1176
    DOI: 10.1039/C7MT00049A

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