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Characterization and lymphocytes proliferation activity of an oligosaccharide degradated from Astragalus polysaccharide

Abstract

An Astragalus oligosaccharide (AOS) degraded from the Astragalus polysaccharide (APS) and purified by membrane dialysis and silicon gel chromatography was studied in this paper. The structural features of AOS were investigated by a combination of chemical and instrumental analysis such as monosaccharide analysis, periodate oxidation-Smith degradation, methylation analysis, electrospray ionization mass spectrometry (ESI-MS), Fourier Transform Infrared Spectrometer (FT-IR) and Nuclear Magnetic Resonance (NMR). The results indicated that AOS is an octasaccharide and that it is consisted of (3→)-linked-Rha, (1→3)-linked-Rha, (1→3,4)-linked-Araf, (1→3)-linked-Gal, terminal-linked Gal and terminal-linked-Glc. The effects of AOS on cyclophosphamide-induced immunosuppression were determined from various aspects, for example, the proliferating of nucleated marrow, red and WBC population, the growth of spleen and thymus and the increasing of hemoglobin(HGB) concentration and granulocyte-macrophage colony stimulating factor (GM-CSF) level. The results indicated that AOS could restore the cyclophosphamide-induced immunosuppression by stimulating the secretion of GM-CSF which promoted the differentiation of progenitor cells after proliferation.

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Publication details

The article was received on 26 Mar 2017, accepted on 09 Jun 2017 and first published on 14 Jun 2017


Article type: Research Article
DOI: 10.1039/C7MD00148G
Citation: Med. Chem. Commun., 2017, Accepted Manuscript
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    Characterization and lymphocytes proliferation activity of an oligosaccharide degradated from Astragalus polysaccharide

    Z. Zhu, J. Zhang, F. Liu, L. Chen, L. Chen and Y. Tang, Med. Chem. Commun., 2017, Accepted Manuscript , DOI: 10.1039/C7MD00148G

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