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Issue 12, 2017
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Methods for monitoring and measurement of protein translation in time and space

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Abstract

Regulation of protein translation constitutes a crucial step in control of gene expression. In comparison to transcriptional regulation, however, translational control has remained a significantly under-studied layer of gene expression. This trend is now beginning to shift thanks to recent advances in next-generation sequencing, proteomics, and microscopy based methodologies which allow accurate monitoring of protein translation rates, from single target messenger RNA molecules to genome-wide scale studies. In this review, we summarize these recent advances, and discuss how they are enabling researchers to study translational regulation in a wide variety of in vitro and in vivo biological systems, with unprecedented depth and spatiotemporal resolution.

Graphical abstract: Methods for monitoring and measurement of protein translation in time and space

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Publication details

The article was received on 31 Jul 2017, accepted on 12 Oct 2017 and first published on 12 Oct 2017


Article type: Review Article
DOI: 10.1039/C7MB00476A
Citation: Mol. BioSyst., 2017,13, 2477-2488
  • Open access: Creative Commons BY license
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    Methods for monitoring and measurement of protein translation in time and space

    M. Dermit, M. Dodel and F. K. Mardakheh, Mol. BioSyst., 2017, 13, 2477
    DOI: 10.1039/C7MB00476A

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