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Tetracycline-controllable artificial microRNA-HOTAIR+EZH2 suppressed the progression of bladder cancer cells

Abstract

Previous studies have suggested that EZH2 was up-regulated in bladder cancer tissues and identified as a biomarker for poor prognosis. However, the biological functions of EZH2 in bladder cancer cells remain unknown. In this research, we discovered that EZH2 expression is irrelevant with the TNM stage and poor prognosis of bladder cancer patients. But suppression of EZH2 can slow the progression of bladder cancer cells. Moreover, we used the technology of synthetic biology to construct the tetracycline-controllable artificial microRNA-HOTAIR+EZH2, which can decrease the expression of HOTAIR and EZH2 in a doxycycline dosage-dependent manner. And we also found that HOTAIR expression was positively correlated with EZH2 expression. Tetracycline-controllable artificial microRNA-HOTAIR+EZH2 can inhibit the proliferation and migration of bladder cancer cells. Meanwhile, the apoptosis rate of bladder cancer cells was increased. Taken together, our research showed the cancer-promoting effects of EZH2 and created a novel method to rescue the development of bladder cancer cells.

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Publication details

The article was received on 05 Apr 2017, accepted on 29 May 2017 and first published on 07 Jun 2017


Article type: Paper
DOI: 10.1039/C7MB00202E
Citation: Mol. BioSyst., 2017, Accepted Manuscript
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    Tetracycline-controllable artificial microRNA-HOTAIR+EZH2 suppressed the progression of bladder cancer cells

    Y. Chen, H. Xie, Y. zou, X. Lai, L. Ma, Y. Liu and J. Li, Mol. BioSyst., 2017, Accepted Manuscript , DOI: 10.1039/C7MB00202E

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