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Dynamic properties of the Growth Hormone Releasing Hormone Receptor (GHRHR) and molecular determinants of GHRH binding

Abstract

The growth hormone-releasing hormone receptor (GHRHR), is a member of the class B GPCR subfamily. GHRH, a 44-residue neuropeptide produced in the hypothalamus, regulates the secretion of growth hormone via its binding to GHRHR. It has recently been associated with several types of cancer such as prostate, breast, pancreatic and ovarian cancer. Family B GPCR peptides bind in a two-step model, where firstly the C-terminal region of the peptide interacts with the extracellular domain (ECD) of the receptor and subsequently, the N-terminal interacts with the seven transmembrane domain (TMD), resulting in activation. Structural information on family B GPCRs is limited; therefore, the use of computational methods may assist their efficient targeting towards new therapeutics. Here, we have utilized several computational tools, such as homology modelling, docking, large-scale molecular dynamics and principal component analysis (PCA), in order to: a) gain information on the dynamic properties of the receptor domains and b) propose a structural model for the interactions between GHRH and the ECD and TMD regions of GHRHR respectively. We conclude that PCA analysis can be used for studying such relative movements in family B GPCRs and provide a structural model, which may assist in the design of highly anticipated non-peptide antagonists against GHRHR.

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Publication details

The article was received on 03 Mar 2017, accepted on 18 May 2017 and first published on 19 May 2017


Article type: Paper
DOI: 10.1039/C7MB00130D
Citation: Mol. BioSyst., 2017, Accepted Manuscript
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    Dynamic properties of the Growth Hormone Releasing Hormone Receptor (GHRHR) and molecular determinants of GHRH binding

    M. Matsoukas and G. A. Spyroulias, Mol. BioSyst., 2017, Accepted Manuscript , DOI: 10.1039/C7MB00130D

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