Metabolic Profiling of asthma in mice and the interventional effects of SPA using Liquid Chromatography and Q-TOF Mass Spectrometry
Asthma is a chronic inflammatory lung disease lead to 250,000 deaths annually. It is need to better understand asthma by identifying new pathogenic molecules. We conducted a liquid-chromatography with time-of-flight mass spectrometer (LC-Q-TOF-MS)-based metabolomics study to intestate of asthma and to investigate the interventional mechanisms of surfactant protein A (SPA) in OVA-induced asthma mice. The results revealed that asthma disturbed 32 metabolites in 9 metabolic pathways. After SPA treatment, the metabolomics profile found in asthma was significantly reversed, shifting much closer to control group, indicating that SPA has therapeutic effects against asthma. Metabolomic pathway analysis by the Ingenuity Pathway Analysis demonstrated that several pathways including fatty acid metabolism, lipid metabolism, purine metabolism were significantly altered in asthma. This study offers new methodologies for the understanding of asthma and the mechanisms of SPA in treating asthma.