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Metabolic profiling study of shikonin's cytotoxic activity in the Huh7 human hepatoma cell line

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Abstract

Shikonin and its enantiomer alkannin, which are natural products, have been extensively studied in vitro and in vivo for, among others, their antitumor activity. The investigation of the molecular pathways involved in their action is of interest, since they are not yet clearly defined. Metabolic profiling in cells can provide a picture of a cell's phenotype upon intervention, assisting in the elucidation of the mechanism of action. In this study, the cytotoxic effect of shikonin on a human hepatocarcinoma cell line was studied. Huh7 cells were treated with shikonin at 5 μM, and it was found that shikonin markedly inhibited cell growth. Metabolic profiling indicated alterations in the metabolic content of the cells and the culture media upon treatment, detecting the metabolic response of the cells. This study demonstrates the potential of metabolomics to improve knowledge on the mechanisms involved in shikonin's antitumor action.

Graphical abstract: Metabolic profiling study of shikonin's cytotoxic activity in the Huh7 human hepatoma cell line

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Publication details

The article was received on 07 Dec 2016, accepted on 14 Feb 2017, published on 07 Mar 2017 and first published online on 07 Mar 2017


Article type: Paper
DOI: 10.1039/C6MB00830E
Citation: Mol. BioSyst., 2017, Advance Article
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    Metabolic profiling study of shikonin's cytotoxic activity in the Huh7 human hepatoma cell line

    E. D. Spyrelli, A. V. Kyriazou, C. Virgiliou, A. Nakas, O. Deda, V. P. Papageorgiou, A. N. Assimopoulou and H. G. Gika, Mol. BioSyst., 2017, Advance Article , DOI: 10.1039/C6MB00830E

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