Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.



Generation and characterization of the blood transcriptome of Macaca thibetana and comparative analysis with M. mulatta

Author affiliations

Abstract

Transcriptome profiles provide a large transcript sequence data set for genomic study, particularly in organisms that have no accurate genome data published. The Tibetan macaque (Macaca thibetana) is commonly considered to be an endemic species to China and an important animal in biomedical research in the present day. In the present study, we report the de novo assembly and characterization of the blood transcriptome of the Tibetan macaque from three individuals, and we also sequenced the blood transcriptome of the rhesus macaque (Macaca mulatta) for comparison. Using RNA-seq technology, 138 million sequencing reads of the M. thibetana transcriptome were generated. The assembly included 327 871 transcripts with an N50 of 1571 bp. According to the sequence similarity search, 80 317 (24.5%) transcripts were annotated in the nr protein database. All transcripts from M. thibetana and M. mulatta were functionally classified and compared using GO and KEGG analyses. The two transcriptomes were different in the GO term of nutrient reservoir activity, and in the KEGG subcategories of signaling molecules and interaction, infectious diseases, cell growth and death, and immune system. The transcriptomes in this study would provide a valuable resource for future functional and comparative genomic studies, and even for biological studies of this non-human primate.

Graphical abstract: Generation and characterization of the blood transcriptome of Macaca thibetana and comparative analysis with M. mulatta

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 13 Nov 2016, accepted on 20 Mar 2017 and first published on 21 Apr 2017


Article type: Paper
DOI: 10.1039/C6MB00771F
Citation: Mol. BioSyst., 2017, Advance Article
  •   Request permissions

    Generation and characterization of the blood transcriptome of Macaca thibetana and comparative analysis with M. mulatta

    P. Li, L. Du, W. Li, Z. Fan, D. Zeng, H. Chen, L. Zhou, Y. Yi, N. Yang, K. Dou, B. Yue and J. Li, Mol. BioSyst., 2017, Advance Article , DOI: 10.1039/C6MB00771F

Search articles by author