Ligand-responsive upregulation of 3′ CITE-mediated translation in a wheat germ cell-free expression system†
Abstract
We have rationally constructed a novel regulation-type of artificial riboswitch that ligand-dose dependently upregulates translation initiation mediated by a 3′ cap-independent translation element (3′ CITE) with no major hybridization switches in a plant expression system (wheat germ extract).