Jump to main content
Jump to site search


Converting Colour to Length Based on Coffee-Ring Effect for Quantitative Immunoassays Using a Ruler as Readout

Abstract

We report a method for converting the colorimetric results of enzyme-link immunosorbent assay (ELISA) into length based on the coffee-ring effect, so that the quantitative detection of analyte can be carried out simply using a ruler. The influence of the shape and lamination of the paper strip to the test results is studied. As a demonstration, human IgG is quantitatively analyzed. It is found that the width of the colored stains is correlated to the concentration of analyte which can be measured for quantitative analysis. The method is promising for quantitative point-of-care detection of biomarkers under resource-limited settings.

Back to tab navigation

Supplementary files

Publication details

The article was received on 18 Oct 2017, accepted on 04 Dec 2017 and first published on 04 Dec 2017


Article type: Communication
DOI: 10.1039/C7LC01127J
Citation: Lab Chip, 2017, Accepted Manuscript
  •   Request permissions

    Converting Colour to Length Based on Coffee-Ring Effect for Quantitative Immunoassays Using a Ruler as Readout

    D. Zhang, B. Gao, Y. Chen and H. liu, Lab Chip, 2017, Accepted Manuscript , DOI: 10.1039/C7LC01127J

Search articles by author

Spotlight

Advertisements