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A planar dielectrophoresis-based chip for high-throughput cell pairing

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Abstract

This paper reports the design and fabrication of a planar chip for high-throughput cell trapping and pairing (more than 2400 single cell–cell pairs in a microwell array) in a 1 × 1.5 cm area by positive dielectrophoresis (p-DEP) within only several minutes. The p-DEP was generated by applying an alternating current signal on a novel two-pair interdigitated array (TPIDA) electrode. The TPIDA electrode not only enabled the planar chip to be incorporated with a most often used PDMS microfluidic channel, but also contributed to a high single cell–cell pairing efficiency up to 74.2% by decreasing the induced electric field during consecutive p-DEP trapping of two cell types. Furthermore, the paired cells in each microwell could be “pushed” together into a microbaffle by a liquid flow through a capillary-sized channel, resulting in single cell–cell contact. More importantly, the planar chip could be used repeatedly by a simple water cleaning process. The planar chip offers an effective way for high-throughput single cell–cell pairing, which could provide a facile platform for cell communication and a precise cell pairing step in cell fusion.

Graphical abstract: A planar dielectrophoresis-based chip for high-throughput cell pairing

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Publication details

The article was received on 09 Oct 2017, accepted on 30 Oct 2017 and first published on 31 Oct 2017


Article type: Communication
DOI: 10.1039/C7LC01082F
Citation: Lab Chip, 2017, Advance Article
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    A planar dielectrophoresis-based chip for high-throughput cell pairing

    C. Wu, R. Chen, Y. Liu, Z. Yu, Y. Jiang and X. Cheng, Lab Chip, 2017, Advance Article , DOI: 10.1039/C7LC01082F

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