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An ultra-small, multi-point, and multi-color photo-detection system with high sensitivity and high dynamic range

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Abstract

Although multi-point, multi-color fluorescence-detection systems are widely used in various sciences, they would find wider applications if they are miniaturized. Accordingly, an ultra-small, four-emission-point and four-color fluorescence-detection system was developed. Its size (space between emission points and a detection plane) is 15 × 10 × 12 mm, which is three-orders-of-magnitude smaller than that of a conventional system. Fluorescence from four emission points with an interval of 1 mm on the same plane was respectively collimated by four lenses and split into four color fluxes by four dichroic mirrors. Then, a total of sixteen parallel color fluxes were directly input into an image sensor and simultaneously detected. The emission-point plane and the detection plane (the image-sensor surface) were parallel and separated by a distance of only 12 mm. The developed system was applied to four-capillary array electrophoresis and successfully achieved Sanger DNA sequencing. Moreover, compared with a conventional system, the developed system had equivalent high fluorescence-detection sensitivity (lower detection limit of 17 pM dROX) and 1.6-orders-of-magnitude higher dynamic range (4.3 orders of magnitude).

Graphical abstract: An ultra-small, multi-point, and multi-color photo-detection system with high sensitivity and high dynamic range

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Publication details

The article was received on 04 Oct 2017, accepted on 23 Oct 2017 and first published on 08 Nov 2017


Article type: Paper
DOI: 10.1039/C7LC01070B
Citation: Lab Chip, 2017, Advance Article
  • Open access: Creative Commons BY-NC license
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    An ultra-small, multi-point, and multi-color photo-detection system with high sensitivity and high dynamic range

    T. Anazawa and M. Yamazaki, Lab Chip, 2017, Advance Article , DOI: 10.1039/C7LC01070B

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