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A multi-throughput multi-organ-on-a-chip system on a plate formatted pneumatic pressure-driven medium circulation platform

Abstract

This paper reports a multi-throughput multi-organ-on-a-chip system formed on a pneumatic pressure-driven medium circulation platform with a microplate-sized format as a novel type of microphysiological system. The pneumatic pressure-driven platform enabled parallelized multi-organ experiments (i.e. simultaneous operation of multiple multi-organ culture units) and pipette-friendly liquid handling for various conventional cell-culture experiments, including cell seeding, medium change, live/dead staining, cell growth analysis, gene expression analysis of collected cells, and liquid chromatography – mass spectrometry analysis of chemical compounds in the culture medium. An eight-throughput two-organ system and a four-throughput four-organ system were constructed on the common platform, with different microfluidic plates. The two-organ system, composed of liver and cancer models, was used to demonstrate the effect of an anticancer prodrug, capecitabine (CAP), whose metabolite 5-fluorouracil (5-FU) after metabolism by HepaRG hepatic cells, inhibited proliferation of HCT-116 cancer cells. The four-organ system, composed of intestine, liver, cancer, and connective tissue models, was used to demonstrate evaluation of the effects of 5-FU and two prodorugs of 5-FU (CAP and tegafur) on multiple organ models, including cancer and connective tissue.

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Publication details

The article was received on 05 Sep 2017, accepted on 11 Nov 2017 and first published on 14 Nov 2017


Article type: Paper
DOI: 10.1039/C7LC00952F
Citation: Lab Chip, 2017, Accepted Manuscript
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    A multi-throughput multi-organ-on-a-chip system on a plate formatted pneumatic pressure-driven medium circulation platform

    T. Satoh, S. Sugiura, K. Shinji, R. Nagasaki, S. Ishida, K. Kikuchi, M. Kakiki and T. Kanamori, Lab Chip, 2017, Accepted Manuscript , DOI: 10.1039/C7LC00952F

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