Jump to main content
Jump to site search


Full Angle Tomographic Phase Microscopy of Flowing Quasi-Spherical Cells

Abstract

We report a reliable full-angle tomographic phase microscopy (FA-TPM) for flowing quasi-spherical cells along microfluidic channels. The method lies on a completely passive optical system, i.e. mechanical scanning or multi-direction probing of the sample is avoided. It exploits the engineered rolling of cells while they are flowing along a microfluidic channel. Here we demonstrate a significant progress in respect to the state of the art of in-flow TPM, by showing a general extension to cells having almost spherical shapes while they are flowing in suspension. In fact, the adopted strategy allows the accurate retrieving of rotation angles through a theoretical model of the cells rotation in dynamic microfluidic flow by matching it with phase-contrast images resulting from holographic reconstructions. So far, the proposed method is the first and the only one that permits to get in-flow TPM by probing the cells with full-angle, achieving accurate 3D refractive index mapping and the simplest optical setup, simultaneously. Experimental proof of concept were performed successfully on human breast adenocarninoma MCF-7 cells, opening the way for the full characterization of circulating tumor cells (CTCs) in the new paradigm of liquid biopsy.

Back to tab navigation

Supplementary files

Publication details

The article was received on 01 Sep 2017, accepted on 31 Oct 2017 and first published on 31 Oct 2017


Article type: Paper
DOI: 10.1039/C7LC00943G
Citation: Lab Chip, 2017, Accepted Manuscript
  •   Request permissions

    Full Angle Tomographic Phase Microscopy of Flowing Quasi-Spherical Cells

    M. M. Villone, P. Memmolo, F. Merola, M. Mugnano, L. Miccio, P. L. Maffettone and P. Ferraro, Lab Chip, 2017, Accepted Manuscript , DOI: 10.1039/C7LC00943G

Search articles by author

Spotlight

Advertisements