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Nanowire sensors monitor bacterial growth kinetics and response to antibiotics

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Abstract

Miniaturized and cost-efficient methods aiming at high throughput analysis of microbes are of great importance for the surveillance and control of infectious diseases and the related issue of antimicrobial resistance. Here we demonstrate a miniature nanosensor based on a honeycomb-patterned silicon nanowire field effect transistor (FET) capable of detection of bacterial growth and antibiotic response in microbiologically relevant nutrient media. We determine the growth kinetics and metabolic state of Escherichia coli cells in undiluted media via the quantification of changes in the source–drain current caused by varying pH values. Furthermore, by measuring the time dependent profile of pH change for bacterial cultures treated with antibiotics, we demonstrate for the first time the possibility of electrically distinguishing between bacteriostatic and bactericidal drug effects. We believe that the use of such nanoscopic FET devices enables addressing parameters that are not easily accessible by conventional optical methods in a label-free format, i.e. monitoring of microbial metabolic activity or stress response.

Graphical abstract: Nanowire sensors monitor bacterial growth kinetics and response to antibiotics

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Publication details

The article was received on 31 Jul 2017, accepted on 19 Oct 2017 and first published on 19 Oct 2017


Article type: Paper
DOI: 10.1039/C7LC00807D
Citation: Lab Chip, 2017, Advance Article
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    Nanowire sensors monitor bacterial growth kinetics and response to antibiotics

    B. Ibarlucea, T. Rim, C. K. Baek, J. A. G. M. de Visser, L. Baraban and G. Cuniberti, Lab Chip, 2017, Advance Article , DOI: 10.1039/C7LC00807D

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