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“Open-top” Microfluidic Device for In-vitro Three-Dimensional Capillary Bed

Abstract

We introduce a novel microfluidic deivce to co-culture the blood vessel network and cell tissues in an in-vivo-like niche. Our “open-top” microfluidic device is composed of microchannels with micropores in the ceiling providing direct fluid access from reservoir to microchannel. Fluid connections through micropores provide novel advantages: i) long-term culture of large-scale microvessel network, ii) different fluid access to inner and exterior side of the microvessel, and iii) co-culturing of microvessel network and small cell tissue. In this study, we have successfully managed to assemble microvessel with 5 mm channel width. Also, we were able to mimick capillary bed condition by co-culturing microvessel and cancer spheroid. Intimate contact between cancer spheroid and microvessel caused vessel recruitment, increase in vessel formation and affected vessel morphology. From all the results, we expect this device to be used as a novel platform for vascularized tissue model.

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Publication details

The article was received on 20 Jun 2017, accepted on 26 Aug 2017 and first published on 08 Sep 2017


Article type: Communication
DOI: 10.1039/C7LC00646B
Citation: Lab Chip, 2017, Accepted Manuscript
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    “Open-top” Microfluidic Device for In-vitro Three-Dimensional Capillary Bed

    S. Oh, H. Ryu, D. Tahk, J. Ko, Y. Chung, H. K. Lee, T. R. Lee and N. L. Jeon, Lab Chip, 2017, Accepted Manuscript , DOI: 10.1039/C7LC00646B

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