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Microfluidics for exosome isolation and analysis: Enabling liquid biopsy for personalized medicine

Abstract

Exosomes, the smallest sized extracellular vesicles (30 ~ 150 nm) packaged with lipids, proteins, functional messenger RNAs and microRNAs, and double-stranded DNA from their cells of origin, have emerged as key players in intercellular communication. Their presence in bodily fluids, where they protect their cargo from degradation, makes them attractive candidates for clinical application as innovative diagnostic and therapeutic tools. But routine isolation and analysis of high purity exosomes in clinical settings is challenging, with conventional methods facing a number of drawbacks including low yield and/or purity, long processing times, high cost, and difficulties in standardization. Here we review a promising solution, microfluidic-based technologies that have incorporated a host of separation and sensing capabilities for exosome isolation, detection, and analysis, with emphasis on point of care and clinical applications. These new capabilities promise to advance fundamental research while paving the way toward routine exosome-based liquid biopsy for personalized medicine.

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Publication details

The article was received on 05 Jun 2017, accepted on 07 Aug 2017 and first published on 10 Aug 2017


Article type: Critical Review
DOI: 10.1039/C7LC00592J
Citation: Lab Chip, 2017, Accepted Manuscript
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    Microfluidics for exosome isolation and analysis: Enabling liquid biopsy for personalized medicine

    J. C. Contreras-Naranjo, H. Wu and V. M. Ugaz, Lab Chip, 2017, Accepted Manuscript , DOI: 10.1039/C7LC00592J

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