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Droplet microfluidics for synthetic biology

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Abstract

Synthetic biology is an interdisciplinary field that aims to engineer biological systems for useful purposes. Organism engineering often requires the optimization of individual genes and/or entire biological pathways (consisting of multiple genes). Advances in DNA sequencing and synthesis have recently begun to enable the possibility of evaluating thousands of gene variants and hundreds of thousands of gene combinations. However, such large-scale optimization experiments remain cost-prohibitive to researchers following traditional molecular biology practices, which are frequently labor-intensive and suffer from poor reproducibility. Liquid handling robotics may reduce labor and improve reproducibility, but are themselves expensive and thus inaccessible to most researchers. Microfluidic platforms offer a lower entry price point alternative to robotics, and maintain high throughput and reproducibility while further reducing operating costs through diminished reagent volume requirements. Droplet microfluidics have shown exceptional promise for synthetic biology experiments, including DNA assembly, transformation/transfection, culturing, cell sorting, phenotypic assays, artificial cells and genetic circuits.

Graphical abstract: Droplet microfluidics for synthetic biology

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Publication details

The article was received on 30 May 2017, accepted on 09 Aug 2017 and first published on 10 Aug 2017


Article type: Critical Review
DOI: 10.1039/C7LC00576H
Citation: Lab Chip, 2017, Advance Article
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    Droplet microfluidics for synthetic biology

    P. C. Gach, K. Iwai, P. W. Kim, N. J. Hillson and A. K. Singh, Lab Chip, 2017, Advance Article , DOI: 10.1039/C7LC00576H

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