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Cell-free extract based optimization of biomolecular circuits with droplet microfluidics

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Abstract

Engineering an efficient biomolecular circuit often requires time-consuming iterations of optimization. Cell-free protein expression systems allow rapid testing of biocircuits in vitro, speeding the design–build–test cycle of synthetic biology. In this paper, we combine this with droplet microfluidics to densely scan a transcription–translation biocircuit space. Our system assays millions of parameter combinations per hour, providing a detailed map of function. The ability to comprehensively map biocircuit parameter spaces allows accurate modeling to predict circuit function and identify optimal circuits and conditions.

Graphical abstract: Cell-free extract based optimization of biomolecular circuits with droplet microfluidics

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Publication details

The article was received on 23 May 2017, accepted on 27 Jul 2017 and first published on 03 Aug 2017


Article type: Communication
DOI: 10.1039/C7LC00552K
Citation: Lab Chip, 2017, Advance Article
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    Cell-free extract based optimization of biomolecular circuits with droplet microfluidics

    Y. Hori, C. Kantak, R. M. Murray and A. R. Abate, Lab Chip, 2017, Advance Article , DOI: 10.1039/C7LC00552K

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