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Issue 13, 2017
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Universal signal generator for dynamic cell stimulation

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Abstract

Dynamic cell stimulation is a powerful technique for probing gene networks and for applications in stem cell differentiation, immunomodulation and signaling. We developed a robust and flexible method and associated microfluidic devices to generate a wide-range of precisely formulated dynamic chemical signals to stimulate live cells and measure their dynamic response. This signal generator is capable of digital to analog conversion (DAC) through combinatoric selection of discrete input concentrations, and outperforms existing methods by both achievable resolution, dynamic range and simplicity in design. It requires no calibration, has minimal space requirements and can be easily integrated into microfluidic cell culture devices. The signal generator hardware and software we developed allows to choose the waveform, period and amplitude of chemical input signals and features addition of well-defined chemical noise to study the role of stochasticity in cellular information processing.

Graphical abstract: Universal signal generator for dynamic cell stimulation

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Publication details

The article was received on 17 May 2017, accepted on 19 May 2017 and first published on 22 May 2017


Article type: Paper
DOI: 10.1039/C7LC00531H
Citation: Lab Chip, 2017,17, 2218-2224
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    Universal signal generator for dynamic cell stimulation

    A. Piehler, N. Ghorashian, C. Zhang and S. Tay, Lab Chip, 2017, 17, 2218
    DOI: 10.1039/C7LC00531H

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