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Issue 15, 2017
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Multifunctional optofluidic lab-on-chip platform for Raman and fluorescence spectroscopic microfluidic analysis

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Abstract

A multifunctional lab-on-a-chip platform for spectroscopic analysis of liquid samples based on an optofluidic jet waveguide is reported. The optofluidic detection scheme is achieved through the total internal reflection arising in a liquid jet of only 150 μm diameter, leading to highly efficient signal excitation and collection. This results in an optofluidic chip with an alignment-free spectroscopic detection scheme, which avoids any background from the sample container. This platform has been designed for multiwavelength fluorescence and Raman spectroscopy. The chip integrates a recirculation system that reduces the required sample volume. The evaluation of the device performance has been accomplished by means of fluorescence measurements performed on eosin Y in water solutions, achieving a limit of detection of 33 pM. The sensor has been applied in Raman spectroscopy of water–ethanol solutions, leading to a limit of detection of 0.18%. As additional application, analysis of riboflavin using fluorescence detection demonstrates the possibility of detecting this vitamin at the 560 pM level (0.21 ng l−1). Although measurements have been performed by means of a compact and low-cost spectrometer, in both cases the micro-jet optofluidic chip achieved similar performances if not better than high-end benchtop based laboratory equipment. This approach paves the way towards portable lab-on-a-chip devices for high sensitivity environmental and biochemical sensing, using optical spectroscopy.

Graphical abstract: Multifunctional optofluidic lab-on-chip platform for Raman and fluorescence spectroscopic microfluidic analysis

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Publication details

The article was received on 27 Apr 2017, accepted on 21 Jun 2017 and first published on 21 Jun 2017


Article type: Paper
DOI: 10.1039/C7LC00460E
Citation: Lab Chip, 2017,17, 2631-2639
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    Multifunctional optofluidic lab-on-chip platform for Raman and fluorescence spectroscopic microfluidic analysis

    G. Persichetti, I. A. Grimaldi, G. Testa and R. Bernini, Lab Chip, 2017, 17, 2631
    DOI: 10.1039/C7LC00460E

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