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Issue 13, 2017
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A simple and highly sensitive spectroscopic fluorescence-detection system for multi-channel plastic-microchip electrophoresis based on side-entry laser-beam zigzag irradiation

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Abstract

A five-color fluorescence-detection system for eight-channel plastic-microchip electrophoresis was developed. In the eight channels (with effective electrophoretic lengths of 10 cm), single-stranded DNA fragments were separated (with single-base resolution up to 300 bases within 10 min), and seventeen-loci STR genotyping for forensic human identification was successfully demonstrated. In the system, a side-entry laser beam is passed through the eight channels (eight A channels), with alternately arrayed seven sacrificial channels (seven B channels), by a technique called “side-entry laser-beam zigzag irradiation.” Laser-induced fluorescence from the eight A channels and Raman-scattered light from the seven B channels are then simultaneously, uniformly, and spectroscopically detected, in the direction perpendicular to the channel array plane, through a transmission grating and a CCD camera. The system is therefore simple and highly sensitive. Because the microchip is fabricated by plastic-injection molding, it is inexpensive and disposable and thus suitable for actual use in various fields.

Graphical abstract: A simple and highly sensitive spectroscopic fluorescence-detection system for multi-channel plastic-microchip electrophoresis based on side-entry laser-beam zigzag irradiation

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Publication details

The article was received on 25 Apr 2017, accepted on 27 May 2017 and first published on 06 Jun 2017


Article type: Paper
DOI: 10.1039/C7LC00448F
Citation: Lab Chip, 2017,17, 2235-2242
  • Open access: Creative Commons BY-NC license
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    A simple and highly sensitive spectroscopic fluorescence-detection system for multi-channel plastic-microchip electrophoresis based on side-entry laser-beam zigzag irradiation

    T. Anazawa, Y. Uchiho, T. Yokoi, G. Chalkidis and M. Yamazaki, Lab Chip, 2017, 17, 2235
    DOI: 10.1039/C7LC00448F

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