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Pre-concentration by liquid intake by paper (P-CLIP): a new technique for large volumes and digital microfluidics

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Abstract

Microfluidic platforms are an attractive option for incorporating complex fluid handling into low-cost and rapid diagnostic tests. A persistent challenge for microfluidics, however, is the mismatch in the “world-to-chip” interface – it is challenging to detect analytes present at low concentrations in systems that can only handle small volumes of sample. Here we describe a new technique termed pre-concentration by liquid intake by paper (P-CLIP) that addresses this mismatch, allowing digital microfluidics to interface with volumes on the order of hundreds of microliters. In P-CLIP, a virtual microchannel is generated to pass a large volume through the device; analytes captured on magnetic particles can be isolated and then resuspended into smaller volumes for further processing and analysis. We characterize this method and demonstrate its utility with an immunoassay for Plasmodium falciparum lactate dehydrogenase, a malaria biomarker, and propose that the P-CLIP strategy may be useful for a wide range of applications that are currently limited by low-abundance analytes.

Graphical abstract: Pre-concentration by liquid intake by paper (P-CLIP): a new technique for large volumes and digital microfluidics

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Publication details

The article was received on 23 Apr 2017, accepted on 02 Jun 2017 and first published on 12 Jun 2017


Article type: Paper
DOI: 10.1039/C7LC00440K
Citation: Lab Chip, 2017, Advance Article
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    Pre-concentration by liquid intake by paper (P-CLIP): a new technique for large volumes and digital microfluidics

    D. G. Rackus, R. P. S. de Campos, C. Chan, M. M. Karcz, B. Seale, T. Narahari, C. Dixon, M. D. Chamberlain and A. R. Wheeler, Lab Chip, 2017, Advance Article , DOI: 10.1039/C7LC00440K

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