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Detection of membrane-bound and soluble antigens by magnetic levitation

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Abstract

Magnetic levitation is a technique for measuring the density and the magnetic properties of objects suspended in a paramagnetic field. We describe a novel magnetic levitation-based method that can specifically detect cell membrane-bound and soluble antigens by measurable changes in levitation height that result from the formation of antibody-coated bead and antigen complex. We demonstrate our method's ability to sensitively detect an array of membrane-bound and soluble antigens found in blood, including T-cell antigen CD3, eosinophil antigen Siglec-8, red blood cell antigens CD35 and RhD, red blood cell-bound Epstein–Barr viral particles, and soluble IL-6, and validate the results by flow cytometry and immunofluorescence microscopy performed in parallel. Additionally, employing an inexpensive, single lens, manual focus, wifi-enabled camera, we extend the portability of our method for its potential use as a point-of-care diagnostic assay.

Graphical abstract: Detection of membrane-bound and soluble antigens by magnetic levitation

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Publication details

The article was received on 12 Apr 2017, accepted on 01 Sep 2017 and first published on 05 Sep 2017


Article type: Paper
DOI: 10.1039/C7LC00402H
Citation: Lab Chip, 2017, Advance Article
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    Detection of membrane-bound and soluble antigens by magnetic levitation

    M. S. Andersen, E. Howard, S. Lu, M. Richard, M. Gregory, G. Ogembo, O. Mazor, P. Gorelik, N. I. Shapiro, A. V. Sharda and I. Ghiran, Lab Chip, 2017, Advance Article , DOI: 10.1039/C7LC00402H

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