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Issue 14, 2017
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An encoded viral micropatch for multiplex cell-based assays through localized gene delivery

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Abstract

The increasing number of potential drug targets and compounds has led to the development of high-throughput cell-based assays. Simultaneous processing of multiple targets in the same experiment based on localized target gene expression is a very efficient strategy for this purpose. To address this need, we present an adenoviral vector-immobilized microparticle with two-dimensional (2D) shape-encoding properties that allows localized patch-like gene delivery to monolayer-cultured cells. This format conveniently achieves multiplexed gene delivery compatible with both high-throughput cellular assays and fluorescence high-content imaging instruments. A multiplex G protein-coupled receptor (GPCR) internalization assay was developed to demonstrate the compatibility of this system with high-throughput image-based cellular assays.

Graphical abstract: An encoded viral micropatch for multiplex cell-based assays through localized gene delivery

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Publication details

The article was received on 05 Apr 2017, accepted on 19 May 2017 and first published on 30 May 2017


Article type: Paper
DOI: 10.1039/C7LC00372B
Citation: Lab Chip, 2017,17, 2435-2442
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    An encoded viral micropatch for multiplex cell-based assays through localized gene delivery

    S. Han, H. J. Bae, S. D. Kim, W. Park and S. Kwon, Lab Chip, 2017, 17, 2435
    DOI: 10.1039/C7LC00372B

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