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Sessile droplets for chemical and biological assays

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Abstract

Sessile droplets are non-movable droplets spanning volumes in the nL-to-μL range. The sessile-droplet-based platform provides a paradigm shift from the conventional, flow-based lab-on-a-chip philosophy, yet offering similar benefits: low reagent/sample consumption, high throughput, automation, and most importantly flexibility and versatility. Moreover, the platform relies less heavily on sophisticated fabrication techniques, often sufficient with a hydrophobic substrate, and no pump is required for operation. In addition, exploiting the physical phenomena that naturally arise when a droplet evaporates, such as the coffee-ring effect or Marangoni flow, can lead to fascinating applications. In this review, we introduce the physics of droplets, and then focus on the different types of chemical and biological assays that have been implemented in sessile droplets, including analyte concentration, particle separation and sorting, cell-based assays, and nucleic acid amplification. Finally, we provide our perspectives on this unique micro-scale platform.

Graphical abstract: Sessile droplets for chemical and biological assays

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Publication details

The article was received on 04 Apr 2017, accepted on 23 May 2017 and first published on 23 May 2017


Article type: Critical Review
DOI: 10.1039/C7LC00366H
Citation: Lab Chip, 2017, Advance Article
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    Sessile droplets for chemical and biological assays

    J. L. Garcia-Cordero and Z. H. Fan, Lab Chip, 2017, Advance Article , DOI: 10.1039/C7LC00366H

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