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Microfluidic-based high-throughput optical trapping of nanoparticles

Abstract

Optical tweezers have emerged as a powerful tool for multiparametric analysis of individual nanoparticles with single-molecule sensitivity. However, its inherent low-throughput characterstic remains a major obstacle for its applications within and beyond the laboratory. This limitation is further exacerbated when working with low concentration nanoparticle samples. Here, we present a microfluidic-based optical tweezers system that can ‘actively’ deliver nanoparticles to a designated microfluidic region for optical trapping and analysis. The active microfluidic delivery of nanoparticles results in significantly improved throughput and efficiency for optical trapping of nanoparticles. We observed a more than tenfold increase of optical trapping throughput for nanoparticles as compared to conventional systems at the same nanoparticle concentration. To demonstrate the utility of this microfluidic-based optical tweezers system, we further used the back-focal plane interferometry coupled with the trapping laser for precise quantitation of nanoparticle size without prior knowledge of the refractive index of nanoparticles. The development of this microfluidic-based active optical tweezers system thus opens the door to high-throughput multiparametric analysis of nanoparticles using precision optical trap in the future.

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Publication details

The article was received on 17 Mar 2017, accepted on 17 May 2017 and first published on 19 May 2017


Article type: Paper
DOI: 10.1039/C7LC00286F
Citation: Lab Chip, 2017, Accepted Manuscript
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    Microfluidic-based high-throughput optical trapping of nanoparticles

    A. Kotnala, Y. Zheng, J. Fu and W. Cheng, Lab Chip, 2017, Accepted Manuscript , DOI: 10.1039/C7LC00286F

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