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Deterministic trapping, encapsulation and retrieval of single-cells

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Abstract

We present a novel method for conducting true single-cell encapsulation at very high efficiency for the manipulation of precious samples. Our unique strategy is based on the sequential capture and original encapsulation of single-cells into a series of hydrodynamic traps. We identified two distinct modes of encapsulation and we established their associated design rules. We improved the trapping scheme to reach a near perfect capture efficiency and make it compatible with the encapsulation process. Finally, we developed the complete device operation that permits highly efficient single-cell encapsulation and droplet retrieval. This platform provides the foundation to a fully integrated multiparameter platform that will impact the analysis of tissues at single-cell resolution.

Graphical abstract: Deterministic trapping, encapsulation and retrieval of single-cells

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Publication details

The article was received on 16 Mar 2017, accepted on 31 May 2017 and first published on 31 May 2017


Article type: Communication
DOI: 10.1039/C7LC00283A
Citation: Lab Chip, 2017, Advance Article
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    Deterministic trapping, encapsulation and retrieval of single-cells

    M. Sauzade and E. Brouzes, Lab Chip, 2017, Advance Article , DOI: 10.1039/C7LC00283A

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