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Issue 17, 2017
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Rapid, label-free CD4 testing using a smartphone compatible device

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Abstract

The most recent guidelines have called for a significant shift towards viral load testing for HIV/AIDS management in developing countries; however point-of-care (POC) CD4 testing still remains an important component of disease staging in multiple developing countries. Advancements in micro/nanotechnologies and consumer electronics have paved the way for mobile healthcare technologies and the development of POC smartphone-based diagnostic assays for disease detection and treatment monitoring. Here, we report a simple, rapid (30 minutes) smartphone-based microfluidic chip for automated CD4 testing using a small volume (30 μL) of whole blood. The smartphone-based device includes an inexpensive (<$5) cell phone accessory and a functionalized disposable microfluidic device. We evaluated the performance of the device using spiked PBS samples and HIV-infected and uninfected whole blood, and compared the microfluidic chip results with the manual analysis and flow cytometry results. Through t-tests, Bland–Altman analyses, and regression tests, we have shown a good agreement between the smartphone-based test and the manual and FACS analysis for CD4 count. The presented technology could have a significant impact on HIV management in developing countries through providing a reliable and inexpensive POC CD4 testing.

Graphical abstract: Rapid, label-free CD4 testing using a smartphone compatible device

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Publication details

The article was received on 15 Mar 2017, accepted on 29 Jun 2017 and first published on 03 Jul 2017


Article type: Paper
DOI: 10.1039/C7LC00273D
Citation: Lab Chip, 2017,17, 2910-2919
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    Rapid, label-free CD4 testing using a smartphone compatible device

    M. K. Kanakasabapathy, H. J. Pandya, M. S. Draz, M. K. Chug, M. Sadasivam, S. Kumar, B. Etemad, V. Yogesh, M. Safavieh, W. Asghar, J. Z. Li, A. M. Tsibris, D. R. Kuritzkes and H. Shafiee, Lab Chip, 2017, 17, 2910
    DOI: 10.1039/C7LC00273D

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