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Issue 13, 2017
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An automated and portable microfluidic chemiluminescence immunoassay for quantitative detection of biomarkers

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Abstract

Microfluidic platforms capable of automated, rapid, sensitive, and quantitative detection of biomarkers from patient samples could make a major impact on clinical or point-of-care (POC) diagnosis. In this work, we realize an automated diagnostic platform composed of two main components: (1) a disposable, self-contained, and integrated microfluidic chip and (2) a portable instrument that carries out completely automated operations. To demonstrate its potential for real-world application, we use injection molding for mass fabrication of the main components of disposable microfluidic chips. The assembled three-layered chip with on-chip mechanical valves for fluid control consists of (1) a top silicone fluidic layer with embedded zigzag microchannels, reagent reservoirs and a negative pressure port, (2) a middle tinfoil layer with patterned antibody/antigen stripes, and (3) a bottom silicone substrate layer with waste reservoirs. The versatility of the microfluidics-based system is demonstrated by implementation of a chemiluminescence immunoassay for quantitative detection of C-reactive protein (CRP) and testosterone in real clinical samples. This lab-on-a-chip platform with features of quantitation, portability and automation provides a promising strategy for POC diagnosis.

Graphical abstract: An automated and portable microfluidic chemiluminescence immunoassay for quantitative detection of biomarkers

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Publication details

The article was received on 08 Mar 2017, accepted on 17 May 2017 and first published on 22 May 2017


Article type: Paper
DOI: 10.1039/C7LC00249A
Citation: Lab Chip, 2017,17, 2225-2234
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    An automated and portable microfluidic chemiluminescence immunoassay for quantitative detection of biomarkers

    B. Hu, J. Li, L. Mou, Y. Liu, J. Deng, W. Qian, J. Sun, R. Cha and X. Jiang, Lab Chip, 2017, 17, 2225
    DOI: 10.1039/C7LC00249A

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