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Rapid real-time recirculating PCR using localized surface plasmon resonance (LSPR) and piezo-electric pumping

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Abstract

Rapid detection and characterization of pathogens in patients with bloodstream infections (BSIs) is a persistent problem for modern medicine, as current techniques are slow or provide incomplete diagnostic information. Real-time polymerase chain reaction (qPCR) allows specific detection of a wide range of targets and quantification of pathogenic burdens to aid in treatment planning. However, new technological advances are required for a rapid and multiplex implementation of qPCR in clinical applications. In this paper, the feasibility of a novel microfluidic platform for qPCR is presented, integrating highly sensitive, label-free localized surface plasmon resonance (LSPR) imaging of DNA hybridization into a recirculating chip design for real-time analysis. Single target and multiplex detection of DNA target amplification are demonstrated, with a limit of detection of 5 fg μL−1 of E. coli DNA for single target PCR, correlating with approximately 300 bacteria per mL. The results of this study demonstrate the potential of this platform for simultaneous real-time detection of multiple target genes within 15 minutes that could provide live saving benefits in patients with BSIs.

Graphical abstract: Rapid real-time recirculating PCR using localized surface plasmon resonance (LSPR) and piezo-electric pumping

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Publication details

The article was received on 28 Feb 2017, accepted on 06 Jul 2017 and first published on 06 Jul 2017


Article type: Paper
DOI: 10.1039/C7LC00211D
Citation: Lab Chip, 2017, Advance Article
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    Rapid real-time recirculating PCR using localized surface plasmon resonance (LSPR) and piezo-electric pumping

    J. M. Haber, P. R. C. Gascoyne and K. Sokolov, Lab Chip, 2017, Advance Article , DOI: 10.1039/C7LC00211D

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